Test methods are presented to measure the antiviral activity of antiviral treated products on plastics and other non-porous surfaces against specific viruses. The official names of this standard are ISO21702: 2019 Measurement of the antiviral activity on plastics and other non-porous surfaces.
Antiviral drugs are commonly used against viral infections and used to control or destroy a virus infection. In other words, antiviral drugs are effective drugs against the virus and prevent the harmful effects of the virus. An antiviral agent means a situation in which the number of infectious viral particles on the surface of a product is reduced. Antiviral agents are agents that reduce the number of infectious viruses on the surface of the product.
Antiviral activity means fighting the virus.
Antibacterial treated consumers widely accept porous and non-porous products with hygienic functions and properties that differ from traditional materials regarding material protection.
ISO 21702 is one of the most sought-after antiviral surface testing methods for assessing the antiviral activity of antiviral treated products such as plastics, coating materials, ceramics, natural leather, artificial leather, and rubber and other non-porous surfaces.
Recently, antiviral treated porous and non-porous products are also on the market. Measurements of antibacterial activity incorporated into non-porous or porous products (fibers) are performed according to ISO 22196 and ISO 20743, respectively.
An ISO 18184: 2014 standard for antiviral activity was incorporated into textile products, which was later replaced by a new version of the standard. The new ISO 21702 standard shows how to test the antiviral activity of non-porous products. As the standard itself indicates, the methods described are based on the standards for antibacterial activity ISO22196 products and antiviral activity ISO18184 products. The ISO21702 standard is intended for application to products such as plastics, coating materials, ceramics, and natural products.
Due to the individual susceptibility of each virus to antiviral agents, the results obtained with one test virus cannot be applied to another pathogen. This standard indicates the use of two viruses. One is the relatively sensitive influenza A (H3N2) virus (A / Hong Kong / 8/68 H3N2), and the other is the more resistant feline calicivirus. The lab uses viruses proposed by different ISO, EN, or ASTM standards (see the list of viruses in this information) or other viruses if available from the culture collection.
The test uses materials with antiviral activity and materials without antiviral activity (negative control). The surface of these fragments is inoculated with a virus suspension, covered with aluminum foil, and incubated at 25 ºC, 90% relative humidity for 24 hours, or within 24 hours if requested by the manufacturer. You should use three for each test group, but you can use more if you want to reduce variability in results, especially for products with low antiviral activity. Tests have shown that the control is used both with and without antivirals to reduce the susceptibility of cells to viral infectivity or viral infectivity.
Quantify the viruses deposited on them as inoculum. After the contact time has elapsed, the deposited virus is collected and quantified to determine, where appropriate, the reduction obtained with the antiviral fragment compared to the non-antiviral component. The results are expressed as the virus titer (amount) per cm of the analyzed sample. This standard does not establish a norm for examining the effectiveness of a product and leaves a measure of consensus among stakeholders.
When we label an antiviral agent, it means that a substance, surface of the material, tissue, or treatment leads to the inactivation of the virus in the sense that it can no longer cause infection.
This is a wide definition and can be caused by various mechanisms, so in actual testing, antivirals are defined as a reduction in disease of sensitive target cells-the virus is specific. On its surface, it can only invade and infect cells that carry essential proteins. The decrease is calculated as a concentration.
These are the primary mechanisms by which antiviral effects can occur. The virus is chemically or physically destroyed
Essential components of the virus are changed.
-Peplomer that cannot initiate cell invasion
-Inhibition of genetic information and viral replication
-Other vital proteins essential in the production cycle are inactivated
-Only for lipid-containing strains such as coronavirus: The lipid barrier is disrupted, allowing significant changes in RNA or protein
Retention: The virus is attached or retained and cannot invade cells and eventually break down.
Masking: The critical sequence for docking to the cell is locked
ISO 21702 is a commonly used surface test for various viruses that can be replaced according to the client’s needs.
The ISO 21702 standard test method is designed to test plastics and other similar materials. ISO 21702 is performed during 24-hour contact with the virus. Standard ISO 21702 antiviral testing is performed using the influenza A virus.
ISO 21702 is one of the demanding antiviral surface test methods for assessing the antibacterial activity of plastics treated with antiviral agents and non-porous products.
Antibacterial plastics can meet the requirements of ISO 21702. However, the treated plastic may not continue to work as expected when adjusted in a simulated environment.
It is important to suitable environmental conditions to the test, such as UV irradiation, humidity, and mold release agents that can affect the effectiveness of antiviral plastics. Durability testing in combination with ISO 21702 or another antibacterial test can provide essential data on the performance of a material after exposure to the conditions found in the intended environment.